New method for laboratory detection of AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae.
نویسندگان
چکیده
A new cefoxitin-agar medium (CAM)-based assay was compared to the previously published modified three-dimensional (M3D) assay for the detection of AmpC production in Escherichia coli and Klebsiella pneumoniae. Clinical isolates of cefoxitin-resistant E. coli (n = 5) and K. pneumoniae (n = 7) and multiple control strains with and without AmpC enzymes were tested by both methods. The CAM method with 4 microg of cefoxitin/ml was equivalent to the M3D method for detecting AmpC production in E. coli and K. pneumoniae. This new method is easier to perform and interpret and allows for testing of multiple isolates on a single plate.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 42 10 شماره
صفحات -
تاریخ انتشار 2004